dna binding dye sample loading reagent (fluidigm)
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Dna Binding Dye Sample Loading Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+binding+dye+sample+loading+reagent/DNA+Binding+Dye/pm41839303-91-119-125
Average 95 stars, based on 265 article reviews
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Real-time Polymerase Chain Reaction:Article Title: Impact of zinc oxide on gut health, immunity, and growth in weaned piglets: exploring potential modes of action Article Snippet: .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 DNA suspension buffer), 3.3 μL of 20 × EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20 × Article Title: Impact of zinc oxide on gut health, immunity, and growth in weaned piglets: exploring potential modes of action Article Snippet: .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 DNA suspension buffer), 3.3 μL of 20 × EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20 × Article Title: Spray-dried porcine plasma improves piglets’ performance and modulates gut immune-related genes in the first week post-weaning Article Snippet: qPCR primer sequences used in this study consisted of both in-house designs and modifications of some primers employed by González-Solé et al [ ], purchased from Condalab, and are listed in . .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 with DNA suspension buffer), 3.3 μL of 20× EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20× Suspension:Article Title: Impact of zinc oxide on gut health, immunity, and growth in weaned piglets: exploring potential modes of action Article Snippet: .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 DNA suspension buffer), 3.3 μL of 20 × EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20 × Article Title: Impact of zinc oxide on gut health, immunity, and growth in weaned piglets: exploring potential modes of action Article Snippet: .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 DNA suspension buffer), 3.3 μL of 20 × EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20 × Article Title: Spray-dried porcine plasma improves piglets’ performance and modulates gut immune-related genes in the first week post-weaning Article Snippet: qPCR primer sequences used in this study consisted of both in-house designs and modifications of some primers employed by González-Solé et al [ ], purchased from Condalab, and are listed in . .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 with DNA suspension buffer), 3.3 μL of 20× EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20× Binding Assay:Article Title: Impact of zinc oxide on gut health, immunity, and growth in weaned piglets: exploring potential modes of action Article Snippet: .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 DNA suspension buffer), 3.3 μL of 20 × EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20 × Article Title: H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts Article Snippet: .. They were analyzed using Universal PCR TaqMan Master Mix (Applied Biosystems), coupled with a Article Title: Impact of zinc oxide on gut health, immunity, and growth in weaned piglets: exploring potential modes of action Article Snippet: .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 DNA suspension buffer), 3.3 μL of 20 × EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20 × Article Title: Spray-dried porcine plasma improves piglets’ performance and modulates gut immune-related genes in the first week post-weaning Article Snippet: qPCR primer sequences used in this study consisted of both in-house designs and modifications of some primers employed by González-Solé et al [ ], purchased from Condalab, and are listed in . .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 with DNA suspension buffer), 3.3 μL of 20× EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20× Article Title: Embryonic Temperature Influences the Mucosal Responses of Atlantic Salmon Alevins to a Bacterial Challenge Article Snippet: After the pre-amplification step, exonuclease I (New England BioLabs) was added to degrade single-stranded oligonucleotide primers at 37 °C for 30 min. 43 μL of TE buffer (Sigma) was added to each sample. .. Each 50-μL cDNA sample was diluted in SsoFast EvaGreen Supermix with Low ROX (Bio-Rad) and 20 × Article Title: Iron absorption and loss, and efficacy of iron supplementation with and without prebiotics in children with virally suppressed HIV: three prospective studies in South Africa Article Snippet: Quantification was carried out with a Nanodrop ND‐1000 Spectrophotometer. (Witec AG) at 260 nm. qPCR was performed with BioMark 96.96 Gene Expression Dynamic Arrays (Fluidigm). .. A sample premix was prepared by mixing 2 μl DNA with 0.4 μl 20 × Article Title: H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts. Article Snippet: .. They were analyzed using Universal PCR TaqMan Master Mix (Applied Biosystems), coupled with a Article Title: Divergent immune dynamics in European perch (Perca fluviatilis): Population-specific responses of peritoneal cells to Aeromonas hydrophila. Article Snippet: The concentration and the purity of the extracted RNA were assessed using a NanoDrop OneC spectrophotometer (NanoDrop Technologies, USA). .. The resulting RNA was reverse-transcribed into cDNA using the Reverse Transcription Master Mix (Standard BioTools, USA) at 42 ◦C for 30 min. Then, cDNA was preamplified using primer pairs (100 μM; Supplementary Table 1) and PreAmp Master Mix (Standard BioTools) in a TAdvanced thermocycler (Biometra, Göttingen, Germany) under the following conditions: 14 cycles of 95 ◦C for 15 s, and 60 ◦C for 4 min. To remove residual single-stranded DNA, primer dimers, and other artifacts, the preamplified cDNA was treated with exonuclease I (New England BioLabs, Ipswich, MA, USA) at 37 ◦C for 30 min. Purified cDNA was diluted in TE buffer (Sigma-Aldrich/Merck, Darmstadt, Germany) and mixed with SsoFast EvaGreen Supermix with Low ROX (BioRad), along with 20 × Polymerase Chain Reaction:Article Title: H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts Article Snippet: .. They were analyzed using Universal PCR TaqMan Master Mix (Applied Biosystems), coupled with a Article Title: H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts. Article Snippet: .. They were analyzed using Universal PCR TaqMan Master Mix (Applied Biosystems), coupled with a Gene Expression:Article Title: Iron absorption and loss, and efficacy of iron supplementation with and without prebiotics in children with virally suppressed HIV: three prospective studies in South Africa Article Snippet: Quantification was carried out with a Nanodrop ND‐1000 Spectrophotometer. (Witec AG) at 260 nm. qPCR was performed with BioMark 96.96 Gene Expression Dynamic Arrays (Fluidigm). .. A sample premix was prepared by mixing 2 μl DNA with 0.4 μl 20 × Reverse Transcription:Article Title: Divergent immune dynamics in European perch (Perca fluviatilis): Population-specific responses of peritoneal cells to Aeromonas hydrophila. Article Snippet: The concentration and the purity of the extracted RNA were assessed using a NanoDrop OneC spectrophotometer (NanoDrop Technologies, USA). .. The resulting RNA was reverse-transcribed into cDNA using the Reverse Transcription Master Mix (Standard BioTools, USA) at 42 ◦C for 30 min. Then, cDNA was preamplified using primer pairs (100 μM; Supplementary Table 1) and PreAmp Master Mix (Standard BioTools) in a TAdvanced thermocycler (Biometra, Göttingen, Germany) under the following conditions: 14 cycles of 95 ◦C for 15 s, and 60 ◦C for 4 min. To remove residual single-stranded DNA, primer dimers, and other artifacts, the preamplified cDNA was treated with exonuclease I (New England BioLabs, Ipswich, MA, USA) at 37 ◦C for 30 min. Purified cDNA was diluted in TE buffer (Sigma-Aldrich/Merck, Darmstadt, Germany) and mixed with SsoFast EvaGreen Supermix with Low ROX (BioRad), along with 20 × Purification:Article Title: Divergent immune dynamics in European perch (Perca fluviatilis): Population-specific responses of peritoneal cells to Aeromonas hydrophila. Article Snippet: The concentration and the purity of the extracted RNA were assessed using a NanoDrop OneC spectrophotometer (NanoDrop Technologies, USA). .. The resulting RNA was reverse-transcribed into cDNA using the Reverse Transcription Master Mix (Standard BioTools, USA) at 42 ◦C for 30 min. Then, cDNA was preamplified using primer pairs (100 μM; Supplementary Table 1) and PreAmp Master Mix (Standard BioTools) in a TAdvanced thermocycler (Biometra, Göttingen, Germany) under the following conditions: 14 cycles of 95 ◦C for 15 s, and 60 ◦C for 4 min. To remove residual single-stranded DNA, primer dimers, and other artifacts, the preamplified cDNA was treated with exonuclease I (New England BioLabs, Ipswich, MA, USA) at 37 ◦C for 30 min. Purified cDNA was diluted in TE buffer (Sigma-Aldrich/Merck, Darmstadt, Germany) and mixed with SsoFast EvaGreen Supermix with Low ROX (BioRad), along with 20 × |
