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dna binding dye sample loading reagent  (fluidigm)


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    Structured Review

    fluidigm dna binding dye sample loading reagent
    Dna Binding Dye Sample Loading Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+binding+dye+sample+loading+reagent/DNA+Binding+Dye/pm41839303-91-119-125
    Average 95 stars, based on 265 article reviews
    dna binding dye sample loading reagent - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Impact of zinc oxide on gut health, immunity, and growth in weaned piglets: exploring potential modes of action
    Article Snippet: .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 DNA suspension buffer), 3.3 μL of 20 × EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20 × DNA Binding Dye Sample Loading Reagent (Fluidigm, PN 100-0388). ..

    Article Title: Impact of zinc oxide on gut health, immunity, and growth in weaned piglets: exploring potential modes of action
    Article Snippet: .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 DNA suspension buffer), 3.3 μL of 20 × EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20 × DNA Binding Dye Sample Loading Reagent (Fluidigm, PN 100-0388). ..

    Article Title: Spray-dried porcine plasma improves piglets’ performance and modulates gut immune-related genes in the first week post-weaning
    Article Snippet: qPCR primer sequences used in this study consisted of both in-house designs and modifications of some primers employed by González-Solé et al [ ], purchased from Condalab, and are listed in . .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 with DNA suspension buffer), 3.3 μL of 20× EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20× DNA Binding Dye Sample Loading Reagent (Fluidigm, PN 100–0388). .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 with DNA suspension buffer), 3.3 μL of 20× EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20× DNA Binding Dye Sample Loading Reagent (Fluidigm, PN 100–0388).

    Suspension:

    Article Title: Impact of zinc oxide on gut health, immunity, and growth in weaned piglets: exploring potential modes of action
    Article Snippet: .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 DNA suspension buffer), 3.3 μL of 20 × EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20 × DNA Binding Dye Sample Loading Reagent (Fluidigm, PN 100-0388). ..

    Article Title: Impact of zinc oxide on gut health, immunity, and growth in weaned piglets: exploring potential modes of action
    Article Snippet: .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 DNA suspension buffer), 3.3 μL of 20 × EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20 × DNA Binding Dye Sample Loading Reagent (Fluidigm, PN 100-0388). ..

    Article Title: Spray-dried porcine plasma improves piglets’ performance and modulates gut immune-related genes in the first week post-weaning
    Article Snippet: qPCR primer sequences used in this study consisted of both in-house designs and modifications of some primers employed by González-Solé et al [ ], purchased from Condalab, and are listed in . .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 with DNA suspension buffer), 3.3 μL of 20× EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20× DNA Binding Dye Sample Loading Reagent (Fluidigm, PN 100–0388). .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 with DNA suspension buffer), 3.3 μL of 20× EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20× DNA Binding Dye Sample Loading Reagent (Fluidigm, PN 100–0388).

    Binding Assay:

    Article Title: Impact of zinc oxide on gut health, immunity, and growth in weaned piglets: exploring potential modes of action
    Article Snippet: .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 DNA suspension buffer), 3.3 μL of 20 × EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20 × DNA Binding Dye Sample Loading Reagent (Fluidigm, PN 100-0388). ..

    Article Title: H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts
    Article Snippet: .. They were analyzed using Universal PCR TaqMan Master Mix (Applied Biosystems), coupled with a DNA Binding Dye Sample Loading Reagent (Fluidigm) and Evagreen (Biotium 31000) in 96.96 Dynamic Arrays on a Biomark System. ..

    Article Title: Impact of zinc oxide on gut health, immunity, and growth in weaned piglets: exploring potential modes of action
    Article Snippet: .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 DNA suspension buffer), 3.3 μL of 20 × EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20 × DNA Binding Dye Sample Loading Reagent (Fluidigm, PN 100-0388). ..

    Article Title: Spray-dried porcine plasma improves piglets’ performance and modulates gut immune-related genes in the first week post-weaning
    Article Snippet: qPCR primer sequences used in this study consisted of both in-house designs and modifications of some primers employed by González-Solé et al [ ], purchased from Condalab, and are listed in . .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 with DNA suspension buffer), 3.3 μL of 20× EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20× DNA Binding Dye Sample Loading Reagent (Fluidigm, PN 100–0388). .. The qPCR sample mix was prepared by combining 2.7 μL of pre-amplified and Exo-I treated samples (diluted 1:20 with DNA suspension buffer), 3.3 μL of 20× EvaGreen DNA Binding Dye (Biotium, PN 31000), and 0.5 μL of 20× DNA Binding Dye Sample Loading Reagent (Fluidigm, PN 100–0388).

    Article Title: Embryonic Temperature Influences the Mucosal Responses of Atlantic Salmon Alevins to a Bacterial Challenge
    Article Snippet: After the pre-amplification step, exonuclease I (New England BioLabs) was added to degrade single-stranded oligonucleotide primers at 37 °C for 30 min. 43 μL of TE buffer (Sigma) was added to each sample. .. Each 50-μL cDNA sample was diluted in SsoFast EvaGreen Supermix with Low ROX (Bio-Rad) and 20 × DNA Binding Dye Sample Loading Reagent (Standard BioTools) to produce the sample mixes. .. After transferring the primer and sample mixes together with one no-template (water) control to the assay and sample inlets, the 48.48-gene expression chips were primed in the MX Controller (Standard BioTools).

    Article Title: Iron absorption and loss, and efficacy of iron supplementation with and without prebiotics in children with virally suppressed HIV: three prospective studies in South Africa
    Article Snippet: Quantification was carried out with a Nanodrop ND‐1000 Spectrophotometer. (Witec AG) at 260 nm. qPCR was performed with BioMark 96.96 Gene Expression Dynamic Arrays (Fluidigm). .. A sample premix was prepared by mixing 2 μl DNA with 0.4 μl 20 × DNA Binding Dye Sample Loading Reagent (PN 100–0388; Fluidigm), 4 μl 2 × Taq Man Gene Expression Master Mix (PN 4359016; Thermo Fisher Scientific), 0.4 μl 20 × EvaGreen DNA binding dye (PN 31000; Biotium), and 1.2 μl Tris‐EDTA buffer. .. We prepared the assay mix by mixing 4 μl of 2 × Assay loading Reagent (PN 85000736; Fluidigm), 0.4 μl Tris‐EDTA buffer, and 3.6 μl of the 20 μmol/l of a forward and reverse primer mix.

    Article Title: H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts.
    Article Snippet: .. They were analyzed using Universal PCR TaqMan Master Mix (Applied Biosystems), coupled with a DNA Binding Dye Sample Loading Reagent (Fluidigm) and Evagreen (Biotium 31000) in 96.96 Dynamic Arrays on a Biomark System. ..

    Article Title: Divergent immune dynamics in European perch (Perca fluviatilis): Population-specific responses of peritoneal cells to Aeromonas hydrophila.
    Article Snippet: The concentration and the purity of the extracted RNA were assessed using a NanoDrop OneC spectrophotometer (NanoDrop Technologies, USA). .. The resulting RNA was reverse-transcribed into cDNA using the Reverse Transcription Master Mix (Standard BioTools, USA) at 42 ◦C for 30 min. Then, cDNA was preamplified using primer pairs (100 μM; Supplementary Table 1) and PreAmp Master Mix (Standard BioTools) in a TAdvanced thermocycler (Biometra, Göttingen, Germany) under the following conditions: 14 cycles of 95 ◦C for 15 s, and 60 ◦C for 4 min. To remove residual single-stranded DNA, primer dimers, and other artifacts, the preamplified cDNA was treated with exonuclease I (New England BioLabs, Ipswich, MA, USA) at 37 ◦C for 30 min. Purified cDNA was diluted in TE buffer (Sigma-Aldrich/Merck, Darmstadt, Germany) and mixed with SsoFast EvaGreen Supermix with Low ROX (BioRad), along with 20 × DNA Binding Dye Sample Loading Reagent (Standard BioTools). .. Expression of the 44 selected immunorelevant target genes and four reference genes (Supplementary Table 1) was analyzed on the BioMark HD System (Standard BioTools).

    Polymerase Chain Reaction:

    Article Title: H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts
    Article Snippet: .. They were analyzed using Universal PCR TaqMan Master Mix (Applied Biosystems), coupled with a DNA Binding Dye Sample Loading Reagent (Fluidigm) and Evagreen (Biotium 31000) in 96.96 Dynamic Arrays on a Biomark System. ..

    Article Title: H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts.
    Article Snippet: .. They were analyzed using Universal PCR TaqMan Master Mix (Applied Biosystems), coupled with a DNA Binding Dye Sample Loading Reagent (Fluidigm) and Evagreen (Biotium 31000) in 96.96 Dynamic Arrays on a Biomark System. ..

    Gene Expression:

    Article Title: Iron absorption and loss, and efficacy of iron supplementation with and without prebiotics in children with virally suppressed HIV: three prospective studies in South Africa
    Article Snippet: Quantification was carried out with a Nanodrop ND‐1000 Spectrophotometer. (Witec AG) at 260 nm. qPCR was performed with BioMark 96.96 Gene Expression Dynamic Arrays (Fluidigm). .. A sample premix was prepared by mixing 2 μl DNA with 0.4 μl 20 × DNA Binding Dye Sample Loading Reagent (PN 100–0388; Fluidigm), 4 μl 2 × Taq Man Gene Expression Master Mix (PN 4359016; Thermo Fisher Scientific), 0.4 μl 20 × EvaGreen DNA binding dye (PN 31000; Biotium), and 1.2 μl Tris‐EDTA buffer. .. We prepared the assay mix by mixing 4 μl of 2 × Assay loading Reagent (PN 85000736; Fluidigm), 0.4 μl Tris‐EDTA buffer, and 3.6 μl of the 20 μmol/l of a forward and reverse primer mix.

    Reverse Transcription:

    Article Title: Divergent immune dynamics in European perch (Perca fluviatilis): Population-specific responses of peritoneal cells to Aeromonas hydrophila.
    Article Snippet: The concentration and the purity of the extracted RNA were assessed using a NanoDrop OneC spectrophotometer (NanoDrop Technologies, USA). .. The resulting RNA was reverse-transcribed into cDNA using the Reverse Transcription Master Mix (Standard BioTools, USA) at 42 ◦C for 30 min. Then, cDNA was preamplified using primer pairs (100 μM; Supplementary Table 1) and PreAmp Master Mix (Standard BioTools) in a TAdvanced thermocycler (Biometra, Göttingen, Germany) under the following conditions: 14 cycles of 95 ◦C for 15 s, and 60 ◦C for 4 min. To remove residual single-stranded DNA, primer dimers, and other artifacts, the preamplified cDNA was treated with exonuclease I (New England BioLabs, Ipswich, MA, USA) at 37 ◦C for 30 min. Purified cDNA was diluted in TE buffer (Sigma-Aldrich/Merck, Darmstadt, Germany) and mixed with SsoFast EvaGreen Supermix with Low ROX (BioRad), along with 20 × DNA Binding Dye Sample Loading Reagent (Standard BioTools). .. Expression of the 44 selected immunorelevant target genes and four reference genes (Supplementary Table 1) was analyzed on the BioMark HD System (Standard BioTools).

    Purification:

    Article Title: Divergent immune dynamics in European perch (Perca fluviatilis): Population-specific responses of peritoneal cells to Aeromonas hydrophila.
    Article Snippet: The concentration and the purity of the extracted RNA were assessed using a NanoDrop OneC spectrophotometer (NanoDrop Technologies, USA). .. The resulting RNA was reverse-transcribed into cDNA using the Reverse Transcription Master Mix (Standard BioTools, USA) at 42 ◦C for 30 min. Then, cDNA was preamplified using primer pairs (100 μM; Supplementary Table 1) and PreAmp Master Mix (Standard BioTools) in a TAdvanced thermocycler (Biometra, Göttingen, Germany) under the following conditions: 14 cycles of 95 ◦C for 15 s, and 60 ◦C for 4 min. To remove residual single-stranded DNA, primer dimers, and other artifacts, the preamplified cDNA was treated with exonuclease I (New England BioLabs, Ipswich, MA, USA) at 37 ◦C for 30 min. Purified cDNA was diluted in TE buffer (Sigma-Aldrich/Merck, Darmstadt, Germany) and mixed with SsoFast EvaGreen Supermix with Low ROX (BioRad), along with 20 × DNA Binding Dye Sample Loading Reagent (Standard BioTools). .. Expression of the 44 selected immunorelevant target genes and four reference genes (Supplementary Table 1) was analyzed on the BioMark HD System (Standard BioTools).



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    H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum <t>(20x),</t> and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.
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    H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum (20x), and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.

    Journal: Frontiers in Immunology

    Article Title: Genetic reinstatement of RIG-I in chickens reveals insights into avian immune evolution and influenza interaction

    doi: 10.3389/fimmu.2025.1680791

    Figure Lengend Snippet: H3N1-challenge experiment confirms the exacerbated disease phenotype of RIG-I and RIG-I-RNF135 chickens infected with virulent avian influenza viruses. The generated transgenic chickens were challenged at 28 weeks of age with H3N1 and assessed for different parameters. (A) Viral shedding based on tracheal swabbing and viral RNA load analysis. (B) Probability of survival in the challenged groups. (C) Expression of RIG-I , RNF135 , and influenza-regulated genes in the duodenum, lung, and spleen. (D) Histological assessment of the reproductive tract; WT-MOCK: normal macroscopical and histological appearance of the salpinx; RNF-RIG-I-H3N1: severe atrophy with mild fibrinous peritonitis in the infundibulum (asterisk), severe lymphoplasmacytic salpingitis (arrowheads) (40x), the magnum (20x), and vasculitis in the magnum vessels (200x). (E) Scoring of lesions in the reproductive tract in all challenged groups, starting with the upper row WT-MOCK, WT-H3N1, RIG-I -H3N1, and RIG-I-RNF135 -H3N1; FP, fibrinous peritonitis; LPS, lymphoplasmacytic salpingitis; LPV, lymphoplasmacytic vasculitis; EYP, egg yolk peritonitis. Error bars indicate standard error of mean (SEM); ( ∗ ) indicate statistical differences between groups tested simultaneously ( p < 0.05). Depending on the normal distribution of the data, multiple group comparison was done either with one-way ANOVA or the Independent-Samples Kruskal-Wallis Test. Data indicate the results of H3N1 in vivo experiment, where the dots represent individual chickens analyzed.

    Article Snippet: The reaction was prepared by mixing 2.5 μl TaqMan Gene Expression Master Mix (Applied Biosystems), 0.25 μl 20X DNA Binding Dye Sample Loading Reagent (Fluidigm), 0.25 μl 20X EvaGreen DNA binding dye (Biotum) and 2 μl of preamplified cDNA.

    Techniques: Infection, Generated, Transgenic Assay, Expressing, Comparison, In Vivo